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KMID : 0043319950180060376
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Archives of Pharmacal Research
1995 Volume.18 No. 6 p.376 ~ p.380
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Colchicine Inhibits Integrin α5β1 Gene Expression during PMA induced dDfferentiation of U937 Cells
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Jang Won-Hee
Lee In-Ja
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Abstract
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Monocyte adhesion involves specific cell surface receptors, integrins and results in cell differentiation. We have studied expression and regulation of integrin . during differntiation of U937 as in vitro model. To determine expression of integrin during differentiation of U937 as in vitro model. To determine expression of integrin genes by RT-PCR (reverse transcription and polymerase chain reaction) method. We determined expression of integrin genes by RT-PCE (reverse transcription and polymerase chain reaction) method. We found that expression of integrin .alpha.5.betha.1 was greatly increased during PMA-induced differentiation of U937 cells and also found that PMA-induced expression of integrin was inhibited by colchicine, microtubule depoly merizing agent. These results indicate that microtubular integrity is associated with expression of integrin. during PMA-induced differentiation of U937 cells.
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KEYWORD
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Colchicine, Microtubule, lntegrin α5β1, RT-PCR
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